Human IDE activation kit by CRISPRa

CAT#: GA102309

IDE CRISPRa kit - CRISPR gene activation of human insulin degrading enzyme

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: IDE
• Locus ID: 3416
• Kit Components:

  GA102309G1, IDE gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA102309G2, IDE gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA102309G3, IDE gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001165946, NM_004969, NM_001322793, NM_001322794, NM_001322795, NM_001322796, NM_001322797, NR_136399
• Synonyms: INSULYSIN
• Summary: 'This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009]'