Human Factor I (CFI) activation kit by CRISPRa

CAT#: GA102318

CFI CRISPRa kit - CRISPR gene activation of human complement factor I

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: CFI
• Locus ID: 3426
• Kit Components:

  GA102318G1, Factor I gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA102318G2, Factor I gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA102318G3, Factor I gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000204, NM_001318057, NM_001331035, N63668
• Synonyms: AHUS3; ARMD13; C3b-INA; C3BINA; FI; IF; KAF
• Summary: 'This gene encodes a serine proteinase that is essential for regulating the complement cascade. The encoded preproprotein is cleaved to produce both heavy and light chains, which are linked by disulfide bonds to form a heterodimeric glycoprotein. This heterodimer can cleave and inactivate the complement components C4b and C3b, and it prevents the assembly of the C3 and C5 convertase enzymes. Defects in this gene cause complement factor I deficiency, an autosomal recessive disease associated with a susceptibility to pyogenic infections. Mutations in this gene have been associated with a predisposition to atypical hemolytic uremic syndrome, a disease characterized by acute renal failure, microangiopathic hemolytic anemia and thrombocytopenia. Primary glomerulonephritis with immune deposits and age-related macular degeneration are other conditions associated with mutations of this gene. [provided by RefSeq, Dec 2015]'