Human GIRK1 (KCNJ3) activation kit by CRISPRa

CAT#: GA102536

KCNJ3 CRISPRa kit - CRISPR gene activation of human potassium voltage-gated channel subfamily J member 3

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: KCNJ3
• Locus ID: 3760
• Kit Components:

  GA102536G1, GIRK1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA102536G2, GIRK1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA102536G3, GIRK1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001260508, NM_001260509, NM_001260510, NM_002239
• Synonyms: GIRK1; KGA; KIR3.1
• Summary: 'Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and plays an important role in regulating heartbeat. It associates with three other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex that also couples to neurotransmitter receptors in the brain and whereby channel activation can inhibit action potential firing by hyperpolarizing the plasma membrane. These multimeric G-protein-gated inwardly-rectifying potassium (GIRK) channels may play a role in the pathophysiology of epilepsy, addiction, Down's syndrome, ataxia, and Parkinson's disease. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, May 2012]'