Human Lactate Dehydrogenase B (LDHB) activation kit by CRISPRa

CAT#: GA102673

LDHB CRISPRa kit - CRISPR gene activation of human lactate dehydrogenase B

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: LDHB
• Locus ID: 3945
• Kit Components:

  GA102673G1, Lactate Dehydrogenase B gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA102673G2, Lactate Dehydrogenase B gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA102673G3, Lactate Dehydrogenase B gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001174097, NM_002300, NM_001315537
• Synonyms: HEL-S-281; LDH-B; LDH-H; LDHBD; TRG-5
• Summary: 'This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13. [provided by RefSeq, Feb 2016]'