Human DNA Ligase IV (LIG4) activation kit by CRISPRa

CAT#: GA102696

LIG4 CRISPRa kit - CRISPR gene activation of human DNA ligase 4

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: LIG4
• Locus ID: 3981
• Kit Components:

  GA102696G1, DNA Ligase IV gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA102696G2, DNA Ligase IV gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA102696G3, DNA Ligase IV gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001098268, NM_002312, NM_206937, NM_001330595, NM_001352598, NM_001352599, NM_001352600, NM_001352601, NM_001352602, NM_001352603, NM_001352604
• Synonyms: LIG4S
• Summary: 'The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]'