Human SMAD1 activation kit by CRISPRa

CAT#: GA102774

SMAD1 CRISPRa kit - CRISPR gene activation of human SMAD family member 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: SMAD1
• Locus ID: 4086
• Kit Components:

  GA102774G1, SMAD1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA102774G2, SMAD1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA102774G3, SMAD1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001003688, NM_005900, NM_001354811, NM_001354812, NM_001354813, NM_001354814, NM_001354816, NM_001354817
• Synonyms: BSP-1; BSP1; JV4-1; JV41; MADH1; MADR1
• Summary: 'The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]'