Human MDMX (MDM4) activation kit by CRISPRa

CAT#: GA102859

MDM4 CRISPRa kit - CRISPR gene activation of human MDM4 regulator of p53

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: MDM4
• Locus ID: 4194
• Kit Components:

  GA102859G1, MDMX gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA102859G2, MDMX gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA102859G3, MDMX gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001204171, NM_001204172, NM_001278516, NM_001278517, NM_001278518, NM_001278519, NM_002393, NR_024171
• Synonyms: HDMX; MDMX; MRP1
• Summary: 'This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2011]'