Human KMT2A activation kit by CRISPRa

CAT#: GA102923

KMT2A CRISPRa kit - CRISPR gene activation of human lysine methyltransferase 2A

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: KMT2A
• Locus ID: 4297
• Kit Components:

  GA102923G1, KMT2A gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA102923G2, KMT2A gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA102923G3, KMT2A gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001197104, NM_005933
• Synonyms: ALL-1; CXXC7; HRX; HTRX1; MLL; MLL/GAS7; MLL1; MLL1A; TET1-MLL; TRX1; WDSTS
• Summary: 'This gene encodes a transcriptional coactivator that plays an essential role in regulating gene expression during early development and hematopoiesis. The encoded protein contains multiple conserved functional domains. One of these domains, the SET domain, is responsible for its histone H3 lysine 4 (H3K4) methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. This protein is processed by the enzyme Taspase 1 into two fragments, MLL-C and MLL-N. These fragments reassociate and further assemble into different multiprotein complexes that regulate the transcription of specific target genes, including many of the HOX genes. Multiple chromosomal translocations involving this gene are the cause of certain acute lymphoid leukemias and acute myeloid leukemias. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2010]'