Human MTH1 (NUDT1) activation kit by CRISPRa

CAT#: GA103013

NUDT1 CRISPRa kit - CRISPR gene activation of human nudix hydrolase 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: NUDT1
• Locus ID: 4521
• Kit Components:

  GA103013G1, MTH1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103013G2, MTH1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103013G3, MTH1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_002452, NM_198948, NM_198949, NM_198950, NM_198952, NM_198953, NM_198954, NM_001367553, NM_001367554, NM_001367555, NR_160264
• Synonyms: MTH1
• Summary: 'Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A rare single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described. [provided by RefSeq, Dec 2018]'