Human smooth muscle Myosin heavy chain 11 (MYH11) activation kit by CRISPRa

CAT#: GA103071

MYH11 CRISPRa kit - CRISPR gene activation of human myosin heavy chain 11

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: MYH11
• Locus ID: 4629
• Kit Components:

  GA103071G1, smooth muscle Myosin heavy chain 11 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103071G2, smooth muscle Myosin heavy chain 11 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103071G3, smooth muscle Myosin heavy chain 11 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001040113, NM_001040114, NM_002474, NM_022844, NM_022870
• Synonyms: AAT4; FAA4; SMHC; SMMHC
• Summary: 'The protein encoded by this gene is a smooth muscle myosin belonging to the myosin heavy chain family. The gene product is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP. The gene encoding a human ortholog of rat NUDE1 is transcribed from the reverse strand of this gene, and its 3' end overlaps with that of the latter. The pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript that encodes a protein consisting of the first 165 residues from the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Alternative splicing generates isoforms that are differentially expressed, with ratios changing during muscle cell maturation. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'