Human NFYB activation kit by CRISPRa

CAT#: GA103202

NFYB CRISPRa kit - CRISPR gene activation of human nuclear transcription factor Y subunit beta

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: NFYB
• Locus ID: 4801
• Kit Components:

  GA103202G1, NFYB gRNA vector 1 in pCas-Guide-CRISPRa

  GA103202G2, NFYB gRNA vector 2 in pCas-Guide-CRISPRa

  GA103202G3, NFYB gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_006166
• Synonyms: CBF-A; CBF-B; HAP3; NF-YB
• Summary: The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq, Jul 2008]