Human PAX6 activation kit by CRISPRa

CAT#: GA103394

PAX6 CRISPRa kit - CRISPR gene activation of human paired box 6

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PAX6
• Locus ID: 5080
• Kit Components:

  GA103394G1, PAX6 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103394G2, PAX6 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103394G3, PAX6 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000280, NM_001127612, NM_001258462, NM_001258463, NM_001258464, NM_001258465, NM_001310158, NM_001310159, NM_001310160, NM_001310161, NM_001604, NM_001368887, NM_001368892, NM_001368894, NM_001368899, NM_001368900, NM_001368903, NM_001368906, NM_001368908, NM_001368909, NM_001368910, NM_001368912, NM_001368915, NM_001368916, NM_001368917, NM_001368918, NM_001368922, NM_001368924, NM_001368926, NM_001368927, NM_001368929, NM_001368888, NM_001368889, NM_001368890, NM_001368891, NM_001368893, NM_001368901, NM_001368902, NM_001368904, NM_001368905, NM_001368907, NM_001368911, NM_001368913, NM_001368914, NM_001368919, NM_001368920, NM_001368921, NM_001368923, NM_001368925, NM_001368928, NM_001368930, NR_160916, NR_160917
• Synonyms: AN; AN2; D11S812E; FVH1; MGDA; WAGR
• Summary: 'This gene encodes paired box protein Pax-6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to a conserved paired box domain, a hallmark feature of this gene family, the encoded protein also contains a homeobox domain. Both domains are known to bind DNA and function as regulators of gene transcription. Activity of this protein is key in the development of neural tissues, particularly the eye. This gene is regulated by multiple enhancers located up to hundreds of kilobases distant from this locus. Mutations in this gene or in the enhancer regions can cause ocular disorders such as aniridia and Peter's anomaly. Use of alternate promoters and alternative splicing results in multiple transcript variants encoding different isoforms. Interestingly, inclusion of a particular alternate coding exon has been shown to increase the length of the paired box domain and alter its DNA binding specificity. Consequently, isoforms that carry the shorter paired box domain regulate a different set of genes compared to the isoforms carrying the longer paired box domain. [provided by RefSeq, Mar 2019]'