Human PEG3 activation kit by CRISPRa

CAT#: GA103473

PEG3 CRISPRa kit - CRISPR gene activation of human paternally expressed 3

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PEG3
• Locus ID: 5178
• Kit Components:

  GA103473G1, PEG3 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103473G2, PEG3 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103473G3, PEG3 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001146184, NM_001146185, NM_001146186, NM_001146187, NM_006210, NM_001369722, NM_001369718, NM_001369720, NM_001369723, NM_001369725, NM_001369728, NM_001369729, NM_001369731, NM_001369732, NM_001369733, NM_001369734, NM_001369738, NR_161475, NR_161476, NM_001369717, NM_001369719, NM_001369721, NM_001369724, NM_001369726, NM_001369727, NM_001369730, NM_001369735, NM_001369736, NM_001369737, NM_001369739
• Synonyms: PW1; ZKSCAN22; ZNF904; ZSCAN24
• Summary: 'In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2009]'