Human PIM1 activation kit by CRISPRa

CAT#: GA103557

PIM1 CRISPRa kit - CRISPR gene activation of human Pim-1 proto-oncogene, serine/threonine kinase

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PIM1
• Locus ID: 5292
• Kit Components:

  GA103557G1, PIM1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103557G2, PIM1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103557G3, PIM1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001243186, NM_002648
• Synonyms: PIM
• Summary: 'The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).[provided by RefSeq, Aug 2011]'