Human Pyruvate Kinase (PKLR) activation kit by CRISPRa

CAT#: GA103574

PKLR CRISPRa kit - CRISPR gene activation of human pyruvate kinase L/R

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PKLR
• Locus ID: 5313
• Kit Components:

  GA103574G1, Pyruvate Kinase gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103574G2, Pyruvate Kinase gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103574G3, Pyruvate Kinase gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000298, NM_181871
• Synonyms: PK1; PKL; PKR; PKRL; RPK
• Summary: 'The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'