Human PKM2 (PKM) activation kit by CRISPRa

CAT#: GA103576

PKM CRISPRa kit - CRISPR gene activation of human pyruvate kinase M1/2

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PKM
• Locus ID: 5315
• Kit Components:

  GA103576G1, PKM2 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103576G2, PKM2 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103576G3, PKM2 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001206796, NM_001206797, NM_001206798, NM_001206799, NM_001316318, NM_002654, NM_182470, NM_182471
• Synonyms: CTHBP; HEL-S-30; OIP3; PK3; PKM2; TCB; THBP1
• Summary: 'This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]'