Human PLOD2 activation kit by CRISPRa

CAT#: GA103611

PLOD2 CRISPRa kit - CRISPR gene activation of human procollagen-lysine,2-oxoglutarate 5-dioxygenase 2

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PLOD2
• Locus ID: 5352
• Kit Components:

  GA103611G1, PLOD2 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103611G2, PLOD2 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103611G3, PLOD2 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000935, NM_182943
• Synonyms: BRKS2; LH2; TLH
• Summary: 'The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]'