Human PPM1A activation kit by CRISPRa

CAT#: GA103700

PPM1A CRISPRa kit - CRISPR gene activation of human protein phosphatase, Mg2+/Mn2+ dependent 1A

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PPM1A
• Locus ID: 5494
• Kit Components:

  GA103700G1, PPM1A gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103700G2, PPM1A gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103700G3, PPM1A gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_021003, NM_177951, NM_177952
• Synonyms: PP2C-ALPHA; PP2CA; PP2Calpha
• Summary: 'The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]'