Human Legumain (LGMN) activation kit by CRISPRa

CAT#: GA103822

LGMN CRISPRa kit - CRISPR gene activation of human legumain

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: LGMN
• Locus ID: 5641
• Kit Components:

  GA103822G1, Legumain gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103822G2, Legumain gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103822G3, Legumain gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001008530, NM_005606, NM_001363699, NM_001363696
• Synonyms: AEP; LGMN1; PRSC1
• Summary: 'This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq, Jul 2008]'