Human RAP1A activation kit by CRISPRa

CAT#: GA104017

RAP1A CRISPRa kit - CRISPR gene activation of human RAP1A, member of RAS oncogene family

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: RAP1A
• Locus ID: 5906
• Kit Components:

  GA104017G1, RAP1A gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA104017G2, RAP1A gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA104017G3, RAP1A gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001010935, NM_001291896, NM_002884, NM_001370216, NM_001370217
• Synonyms: C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21
• Summary: 'This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]'