Human RASA1 activation kit by CRISPRa

CAT#: GA104031

RASA1 CRISPRa kit - CRISPR gene activation of human RAS p21 protein activator 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: RASA1
• Locus ID: 5921
• Kit Components:

  GA104031G1, RASA1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA104031G2, RASA1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA104031G3, RASA1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_002890, NM_022650
• Synonyms: CM-AVM; CMAVM; GAP; p120; p120GAP; p120RASGAP; PKWS; RASA; RASGAP
• Summary: 'The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues. [provided by RefSeq, May 2012]'