Human UPF1 activation kit by CRISPRa

CAT#: GA104070

UPF1 CRISPRa kit - CRISPR gene activation of human UPF1 RNA helicase and ATPase

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: UPF1
• Locus ID: 5976
• Kit Components:

  GA104070G1, UPF1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA104070G2, UPF1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA104070G3, UPF1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001297549, NM_002911
• Synonyms: HUPF1; NORF1; pNORF1; RENT1; smg-2
• Summary: 'This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]'