Human SHC (SHC1) activation kit by CRISPRa

CAT#: GA104400

SHC1 CRISPRa kit - CRISPR gene activation of human SHC adaptor protein 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: SHC1
• Locus ID: 6464
• Kit Components:

  GA104400G1, SHC gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA104400G2, SHC gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA104400G3, SHC gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001130040, NM_001130041, NM_001202859, NM_003029, NM_183001
• Synonyms: SHC; SHCA
• Summary: 'This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]'