Human Melanoma gp100 (PMEL) activation kit by CRISPRa

CAT#: GA104416

PMEL CRISPRa kit - CRISPR gene activation of human premelanosome protein

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PMEL
• Locus ID: 6490
• Kit Components:

  GA104416G1, Melanoma gp100 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA104416G2, Melanoma gp100 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA104416G3, Melanoma gp100 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001200053, NM_001200054, NM_001320121, NM_001320122, NM_006928
• Synonyms: D12S53E; gp100; ME20; ME20-M; ME20M; P1; P100; PMEL17; SI; SIL; SILV
• Summary: 'This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011]'