Human SLC12A1 activation kit by CRISPRa

CAT#: GA104477

SLC12A1 CRISPRa kit - CRISPR gene activation of human solute carrier family 12 member 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: SLC12A1
• Locus ID: 6557
• Kit Components:

  GA104477G1, SLC12A1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA104477G2, SLC12A1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA104477G3, SLC12A1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000338, NM_001184832
• Synonyms: BSC1; NKCC2
• Summary: 'This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their biological validity in humans has not been experimentally proven.[provided by RefSeq, May 2010]'