Human SLC12A3 activation kit by CRISPRa

CAT#: GA104479

SLC12A3 CRISPRa kit - CRISPR gene activation of human solute carrier family 12 member 3

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: SLC12A3
• Locus ID: 6559
• Kit Components:

  GA104479G1, SLC12A3 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA104479G2, SLC12A3 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA104479G3, SLC12A3 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000339, NM_001126107, NM_001126108
• Synonyms: NCC; NCCT; TSC
• Summary: 'This gene encodes a renal thiazide-sensitive sodium-chloride cotransporter that is important for electrolyte homeostasis. This cotransporter mediates sodium and chloride reabsorption in the distal convoluted tubule. Mutations in this gene cause Gitelman syndrome, a disease similar to Bartter's syndrome, that is characterized by hypokalemic alkalosis combined with hypomagnesemia, low urinary calcium, and increased renin activity associated with normal blood pressure. This cotransporter is the target for thiazide diuretics that are used for treating high blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'