Human SNAP25 activation kit by CRISPRa

CAT#: GA104529

SNAP25 CRISPRa kit - CRISPR gene activation of human synaptosome associated protein 25

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: SNAP25
• Locus ID: 6616
• Kit Components:

  GA104529G1, SNAP25 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA104529G2, SNAP25 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA104529G3, SNAP25 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_003081, NM_130811, NM_001322902, NM_001322903, NM_001322904, NM_001322905, NM_001322906, NM_001322907, NM_001322908, NM_001322909, NM_001322910
• Synonyms: bA416N4.2; dJ1068F16.2; RIC-4; RIC4; SEC9; SNAP; SNAP-25
• Summary: 'Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]'