Human ELOC activation kit by CRISPRa

CAT#: GA104775

ELOC CRISPRa kit - CRISPR gene activation of human elongin C

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: ELOC
• Locus ID: 6921
• Kit Components:

  GA104775G1, ELOC gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA104775G2, ELOC gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA104775G3, ELOC gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001204857, NM_001204858, NM_001204859, NM_001204860, NM_001204861, NM_001204862, NM_001204863, NM_001204864, NM_005648
• Synonyms: eloC; SIII
• Summary: 'This gene encodes the protein elongin C, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been identified. [provided by RefSeq, Mar 2011]'