Human TCF3 / E2A (TCF3) activation kit by CRISPRa

CAT#: GA104782

TCF3 CRISPRa kit - CRISPR gene activation of human transcription factor 3

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: TCF3
• Locus ID: 6929
• Kit Components:

  GA104782G1, TCF3 / E2A gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA104782G2, TCF3 / E2A gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA104782G3, TCF3 / E2A gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001136139, NM_003200, NM_001351778, NM_001351779
• Synonyms: AGM8; bHLHb21; E2A; E47; ITF1; TCF-3; VDIR
• Summary: 'This gene encodes a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may play a role in lymphoid malignancies. This gene is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1;19), with PBX1), childhood leukemia (t(19;19), with TFPT) and acute leukemia (t(12;19), with ZNF384). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, Sep 2011]'