Human Thymine DNA glycosylase (TDG) activation kit by CRISPRa

CAT#: GA104810

TDG CRISPRa kit - CRISPR gene activation of human thymine DNA glycosylase

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: TDG
• Locus ID: 6996
• Kit Components:

  GA104810G1, Thymine DNA glycosylase gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA104810G2, Thymine DNA glycosylase gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA104810G3, Thymine DNA glycosylase gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001008411, NM_003211, NM_001363612
• Synonyms: E130317C12Rik; JZA-3; Jza1; OTTMUSP00000028912; OTTMUSP00000028913; thymine DNA glycosylase
• Summary: 'The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq, Jul 2008]'