Human Troponin I fast skeletal muscle (TNNI2) activation kit by CRISPRa

CAT#: GA104928

TNNI2 CRISPRa kit - CRISPR gene activation of human troponin I2, fast skeletal type

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: TNNI2
• Locus ID: 7136
• Kit Components:

  GA104928G1, Troponin I fast skeletal muscle gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA104928G2, Troponin I fast skeletal muscle gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA104928G3, Troponin I fast skeletal muscle gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001145829, NM_001145841, NM_003282
• Synonyms: AMCD2B; DA2B; FSSV; fsTnI
• Summary: 'This gene encodes a fast-twitch skeletal muscle protein, a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible for the calcium-dependent regulation of striated muscle contraction. Mouse studies show that this component is also present in vascular smooth muscle and may play a role in regulation of smooth muscle function. In addition to muscle tissues, this protein is found in corneal epithelium, cartilage where it is an inhibitor of angiogenesis to inhibit tumor growth and metastasis, and mammary gland where it functions as a co-activator of estrogen receptor-related receptor alpha. This protein also suppresses tumor growth in human ovarian carcinoma. Mutations in this gene cause myopathy and distal arthrogryposis type 2B. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]'