Human Troponin T1 (TNNT1) activation kit by CRISPRa

CAT#: GA104930

TNNT1 CRISPRa kit - CRISPR gene activation of human troponin T1, slow skeletal type

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: TNNT1
• Locus ID: 7138
• Kit Components:

  GA104930G1, Troponin T1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA104930G2, Troponin T1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA104930G3, Troponin T1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001126132, NM_001126133, NM_001291774, NM_003283
• Synonyms: ANM; NEM5; STNT; TNT; TNTS
• Summary: 'This gene encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'