Human Hamartin (TSC1) activation kit by CRISPRa

CAT#: GA104986

TSC1 CRISPRa kit - CRISPR gene activation of human TSC complex subunit 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: TSC1
• Locus ID: 7248
• Kit Components:

  GA104986G1, Hamartin gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA104986G2, Hamartin gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA104986G3, Hamartin gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000368, NM_001008567, NM_001162426, NM_001162427, N63914, NM_001362177
• Synonyms: LAM; TSC
• Summary: 'This gene is a tumor suppressor gene that encodes the growth inhibitory protein hamartin. The encoded protein interacts with and stabilizes the GTPase activating protein tuberin. This hamartin-tuberin complex negatively regulates mammalian target of rapamycin complex 1 (mTORC1) signalling which is a major regulator of anabolic cell growth. This protein also functions as a co-chaperone for Hsp90 that inhibits its ATPase activity. This protein functions as a facilitator of Hsp90-mediated folding of kinase and non-kinase clients, including Tsc2 and thereby preventing their ubiquitination and proteasomal degradation. Mutations in this gene have been associated with tuberous sclerosis. [provided by RefSeq, Apr 2018]'