Human Von Hippel Lindau (VHL) activation kit by CRISPRa

CAT#: GA105119

VHL CRISPRa kit - CRISPR gene activation of human von Hippel-Lindau tumor suppressor

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: VHL
• Locus ID: 7428
• Kit Components:

  GA105119G1, Von Hippel Lindau gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA105119G2, Von Hippel Lindau gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA105119G3, Von Hippel Lindau gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000551, NM_198156, NM_001354723
• Synonyms: HRCA1; pVHL; RCA1; VHL1
• Summary: 'Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]'