Human Bestrophin (BEST1) activation kit by CRISPRa

CAT#: GA105127

BEST1 CRISPRa kit - CRISPR gene activation of human bestrophin 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: BEST1
• Locus ID: 7439
• Kit Components:

  GA105127G1, Bestrophin gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA105127G2, Bestrophin gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA105127G3, Bestrophin gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001139443, NM_001300786, NM_001300787, NM_004183, NR_134580, NM_001363592, NM_001363593, NM_001363591
• Synonyms: ARB; BEST; BMD; RP50; TU15B; VMD2
• Summary: 'This gene encodes a member of the bestrophin gene family. This small gene family is characterized by proteins with a highly conserved N-terminus with four to six transmembrane domains. Bestrophins may form chloride ion channels or may regulate voltage-gated L-type calcium-ion channels. Bestrophins are generally believed to form calcium-activated chloride-ion channels in epithelial cells but they have also been shown to be highly permeable to bicarbonate ion transport in retinal tissue. Mutations in this gene are responsible for juvenile-onset vitelliform macular dystrophy (VMD2), also known as Best macular dystrophy, in addition to adult-onset vitelliform macular dystrophy (AVMD) and other retinopathies. Alternative splicing results in multiple variants encoding distinct isoforms.[provided by RefSeq, Nov 2008]'