Human 14-3-3 epsilon (YWHAE) activation kit by CRISPRa

CAT#: GA105182

YWHAE CRISPRa kit - CRISPR gene activation of human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: YWHAE
• Locus ID: 7531
• Kit Components:

  GA105182G1, 14-3-3 epsilon gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA105182G2, 14-3-3 epsilon gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA105182G3, 14-3-3 epsilon gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_006761, NR_024058
• Synonyms: 14-3-3E; HEL2; KCIP-1; MDCR; MDS
• Summary: 'This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008]'