Human ZAP70 activation kit by CRISPRa

CAT#: GA105186

ZAP70 CRISPRa kit - CRISPR gene activation of human zeta chain of T cell receptor associated protein kinase 70

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: ZAP70
• Locus ID: 7535
• Kit Components:

  GA105186G1, ZAP70 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA105186G2, ZAP70 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA105186G3, ZAP70 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001079, NM_207519
• Synonyms: SRK; STCD; STD; TZK; ZAP-70
• Summary: 'This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'