Human splicing factor 1 (SF1) activation kit by CRISPRa

CAT#: GA105187

SF1 CRISPRa kit - CRISPR gene activation of human splicing factor 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: SF1
• Locus ID: 7536
• Kit Components:

  GA105187G1, splicing factor 1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA105187G2, splicing factor 1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA105187G3, splicing factor 1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001178030, NM_001178031, NM_004630, NM_201995, NM_201997, NM_201998, NR_033649, NR_033650, NM_001346363, NM_001346364, NM_001346409, NM_001346410
• Synonyms: BBP; D11S636; MBBP; ZCCHC25; ZFM1; ZNF162
• Summary: 'This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]'