Human ELL activation kit by CRISPRa

CAT#: GA105430

ELL CRISPRa kit - CRISPR gene activation of human elongation factor for RNA polymerase II

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: ELL
• Locus ID: 8178
• Kit Components:

  GA105430G1, ELL gRNA vector 1 in pCas-Guide-CRISPRa

  GA105430G2, ELL gRNA vector 2 in pCas-Guide-CRISPRa

  GA105430G3, ELL gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_006532
• Synonyms: C19orf17; ELL1; MEN; PPP1R68
• Summary: Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Elongation factor component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (PubMed:22195968, PubMed:23932780). Specifically required for stimulating the elongation step of RNA polymerase II- and III-dependent snRNA gene transcription (PubMed:23932780). ELL also plays an early role before its assembly into in the SEC complex by stabilizing RNA polymerase II recruitment/initiation and entry into the pause site. Required to stabilize the pre-initiation complex and early elongation. [UniProtKB/Swiss-Prot Function]