Human SMC1 (SMC1A) activation kit by CRISPRa

CAT#: GA105456

SMC1A CRISPRa kit - CRISPR gene activation of human structural maintenance of chromosomes 1A

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: SMC1A
• Locus ID: 8243
• Kit Components:

  GA105456G1, SMC1A gRNA vector 1 in pCas-Guide-CRISPRa

  GA105456G2, SMC1A gRNA vector 2 in pCas-Guide-CRISPRa

  GA105456G3, SMC1A gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001281463, NM_006306
• Synonyms: CDLS2; DXS423E; SB1.8; SMC1; SMC1alpha; SMC1L1; SMCB
• Summary: Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]