Human CDC45L (CDC45) activation kit by CRISPRa

CAT#: GA105484

CDC45 CRISPRa kit - CRISPR gene activation of human cell division cycle 45

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: CDC45
• Locus ID: 8318
• Kit Components:

  GA105484G1, CDC45 gRNA vector 1 in pCas-Guide-CRISPRa

  GA105484G2, CDC45 gRNA vector 2 in pCas-Guide-CRISPRa

  GA105484G3, CDC45 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001178010, NM_001178011, NM_003504, NM_001369291, NR_161281
• Synonyms: CDC45L; CDC45L2; PORC-PI-1
• Summary: The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]