Human SynGAP (SYNGAP1) activation kit by CRISPRa

CAT#: GA105873

SYNGAP1 CRISPRa kit - CRISPR gene activation of human synaptic Ras GTPase activating protein 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: SYNGAP1
• Locus ID: 8831
• Kit Components:

  GA105873G1, SYNGAP1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA105873G2, SYNGAP1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA105873G3, SYNGAP1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_006772, NM_001130066
• Synonyms: MRD5; RASA1; RASA5; SYNGAP
• Summary: This gene encodes a Ras GTPase activating protein that is a member of the N-methyl-D-aspartate receptor complex. The N-terminal domain of the protein contains a Ras-GAP domain, a pleckstrin homology domain, and a C2 domain that may be involved in binding of calcium and phospholipids. The C-terminal domain consists of a ten histidine repeat region, serine and tyrosine phosphorylation sites, and a T/SXV motif required for postsynaptic scaffold protein interaction. The encoded protein negatively regulates Ras, Rap and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor trafficking to the postsynaptic membrane to regulate synaptic plasticity and neuronal homeostasis. Allelic variants of this gene are associated with intellectual disability and autism spectrum disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016]