Human p35 (CDK5R1) activation kit by CRISPRa

CAT#: GA105890

CDK5R1 CRISPRa kit - CRISPR gene activation of human cyclin dependent kinase 5 regulatory subunit 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: CDK5R1
• Locus ID: 8851
• Kit Components:

  GA105890G1, CDK5R1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA105890G2, CDK5R1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA105890G3, CDK5R1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_003885
• Synonyms: CDK5P35; CDK5R; NCK5A; p23; p25; p35; p35nck5a
• Summary: The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease. [provided by RefSeq, Jul 2008]