Human TOP3B activation kit by CRISPRa

CAT#: GA105959

TOP3B CRISPRa kit - CRISPR gene activation of human DNA topoisomerase III beta

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: TOP3B
• Locus ID: 8940
• Kit Components:

  GA105959G1, TOP3B gRNA vector 1 in pCas-Guide-CRISPRa

  GA105959G2, TOP3B gRNA vector 2 in pCas-Guide-CRISPRa

  GA105959G3, TOP3B gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001282112, NM_001282113, NM_003935, NM_001349845, NM_001349847, NM_001349848, NM_001349850, NM_001349851, NM_001349852, NR_146277
• Synonyms: TOP3B1
• Summary: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of this gene may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Aug 2013]