Human UCP4 (SLC25A27) activation kit by CRISPRa

CAT#: GA106337

SLC25A27 CRISPRa kit - CRISPR gene activation of human solute carrier family 25 member 27

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: SLC25A27
• Locus ID: 9481
• Kit Components:

  GA106337G1, SLC25A27 gRNA vector 1 in pCas-Guide-CRISPRa

  GA106337G2, SLC25A27 gRNA vector 2 in pCas-Guide-CRISPRa

  GA106337G3, SLC25A27 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001204051, NM_001204052, NM_004277
• Synonyms: UCP4
• Summary: Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. Transcripts of this gene are only detected in brain tissue and are specifically modulated by various environmental conditions. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011]