Human H2AFY activation kit by CRISPRa

CAT#: GA106396

H2AFY CRISPRa kit - CRISPR gene activation of human H2A histone family member Y

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: H2AFY
• Locus ID: 9555
• Kit Components:

  GA106396G1, H2AFY gRNA vector 1 in pCas-Guide-CRISPRa

  GA106396G2, H2AFY gRNA vector 2 in pCas-Guide-CRISPRa

  GA106396G3, H2AFY gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001040158, NM_004893, NM_138609, NM_138610
• Synonyms: H2A.y; H2A/y; H2AF12M; MACROH2A1.1; macroH2A1.2; mH2A1
• Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2015]