Human HS3ST3B1 activation kit by CRISPRa

CAT#: GA106734

HS3ST3B1 CRISPRa kit - CRISPR gene activation of human heparan sulfate-glucosamine 3-sulfotransferase 3B1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: HS3ST3B1
• Locus ID: 9953
• Kit Components:

  GA106734G1, HS3ST3B1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA106734G2, HS3ST3B1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA106734G3, HS3ST3B1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_006041, NR_130138
• Synonyms: 3-OST-3B; 3OST3B1; h3-OST-3B
• Summary: The protein encoded by this gene is a type II integral membrane protein that belongs to the 3-O-sulfotransferases family. These proteins catalyze the addition of sulfate groups at the 3-OH position of glucosamine in heparan sulfate. The substrate specificity of individual members of the family is based on prior modification of the heparan sulfate chain, thus allowing different members of the family to generate binding sites for different proteins on the same heparan sulfate chain. Following treatment with a histone deacetylase inhibitor, expression of this gene is activated in a pancreatic cell line. The increased expression results in promotion of the epithelial-mesenchymal transition. In addition, the modification catalyzed by this protein allows herpes simplex virus membrane fusion and penetration. A very closely related homolog with an almost identical sulfotransferase domain maps less than 1 Mb away. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]