Human Kaiso (ZBTB33) activation kit by CRISPRa

CAT#: GA106778

ZBTB33 CRISPRa kit - CRISPR gene activation of human zinc finger and BTB domain containing 33

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: ZBTB33
• Locus ID: 10009
• Kit Components:

  GA106778G1, ZBTB33 gRNA vector 1 in pCas-Guide-CRISPRa

  GA106778G2, ZBTB33 gRNA vector 2 in pCas-Guide-CRISPRa

  GA106778G3, ZBTB33 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001184742, NM_006777
• Synonyms: ZNF-kaiso; ZNF348
• Summary: This gene encodes a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway, and may also activate transcription of a subset of target genes by the recruitment of catenin delta-2 (CTNND2). Its interaction with catenin delta-1 (CTNND1) inhibits binding to both methylated and non-methylated DNA. It also interacts directly with the nuclear import receptor Importin-α2 (also known as karyopherin alpha2 or RAG cohort 1), which may mediate nuclear import of this protein. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, May 2010]