Human ALIX (PDCD6IP) activation kit by CRISPRa

CAT#: GA106783

PDCD6IP CRISPRa kit - CRISPR gene activation of human programmed cell death 6 interacting protein

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PDCD6IP
• Locus ID: 10015
• Kit Components:

  GA106783G1, PDCD6IP gRNA vector 1 in pCas-Guide-CRISPRa

  GA106783G2, PDCD6IP gRNA vector 2 in pCas-Guide-CRISPRa

  GA106783G3, PDCD6IP gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001162429, NM_001256192, NM_013374, NR_027867, NR_027868
• Synonyms: AIP1; ALIX; DRIP4; HP95
• Summary: This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012]