Human TRIB1 activation kit by CRISPRa

CAT#: GA106951

TRIB1 CRISPRa kit - CRISPR gene activation of human tribbles pseudokinase 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: TRIB1
• Locus ID: 10221
• Kit Components:

  GA106951G1, TRIB1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA106951G2, TRIB1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA106951G3, TRIB1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001282985, NM_025195
• Synonyms: C8FW; GIG-2; GIG2; SKIP1; TRB-1; TRB1
• Summary: Adapter protein involved in protein degradation by interacting with RFWD2/COP1 ubiquitin ligase (PubMed:27041596). The RFWD2-binding motif is masked by autoinhibitory interactions with the protein kinase domain (PubMed:26455797). Serves to alter RFWD2 substrate specificity by directing the activity of RFWD2 toward CEBPA (PubMed:27041596). Binds selectively the recognition sequence of CEBPA (PubMed:26455797). Regulates myeloid cell differentiation by altering the expression of CEBPA in a RFWD2-dependent manner (By similarity). Controls macrophage, eosinophil and neutrophil differentiation via the COP1-binding domain (By similarity). Interacts with MAPK kinases and regulates activation of MAP kinases, but has no kinase activity (PubMed:15299019, PubMed:26455797). [UniProtKB/Swiss-Prot Function]